Part:BBa_K277031
3L.3_23.B1.05
3L.3_23.B1.05 is 706 bases long and is cloned into the pGem-T vector.
3L.3_23.B1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.05 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
mutation_affecting_coding_sequence YCL054W_re_remove_MmeI (32..43) removal of MmeI
reverse_primer YCL054W_tagr2v1 (124..151)
gene YCL054W (-484..+2041) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants
Sequence (the first 706 bases correspond to coordinates 22137..22842 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 90
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 90
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 378
Illegal BamHI site found at 139
Illegal BamHI site found at 154 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 90
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 90
- 1000COMPATIBLE WITH RFC[1000]
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